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. Author manuscript; available in PMC: 2007 Jun 15.
Published in final edited form as: Free Radic Biol Med. 2006 Mar 9;40(12):2147–2154. doi: 10.1016/j.freeradbiomed.2006.02.010

Figure 2. Culture media pO2 and HIF activity in response to changes in ambient O2.

Figure 2

Plates containing cells in culture were moved from 20% O2 ambience to 0.5% O2 ambience. Dissolved O2 concentration in the culture media was determined after 24h of maintenance in 20% or 0.5% as indicated (A). B. The hypoxic exposure did not influence cell viability. C&D, Cells maintained in 20% O2 ambience were plated and moved to 0.5% O2 ambience where they were cultured for the specified duration. HIF activity subsided over time (C) while total protein content (D) in the cells were not affected. *, p<0.05. lower (A) or higher (C) compared to the paired control group maintained in 20% O2. †, p<0.05; lower compared to the HIF activity in the 0.5% group of the immediately previous time point. Results are mean ± S.D.