Figure 3. Inducible HIF activity is dependent on the O2 ambience to which the cells are adjusted to and not on absolute pO2.
A. Cells were plated and maintained in 30% O2 ambience for 24h. After this period, cells were either moved to 20% or 30% ambience as indicated. After another 24h of culture, luciferase activity was measured. B. The said changes in O2 ambience did not influence cell viability (also see Fig. 2B). Results are mean ± S.D. *, p<0.05.