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. Author manuscript; available in PMC: 2007 Jun 15.
Published in final edited form as: Free Radic Biol Med. 2006 Mar 9;40(12):2147–2154. doi: 10.1016/j.freeradbiomed.2006.02.010

Figure 7. Knock-down of prolyl hydroxylase (PHD) 1–3 expression elevates HIF activity.

Figure 7

Cells maintained in 20% O2 ambience were plated in antibiotic-free culture media for 24h before transfection. Cells were transfected with 100 nM per sample with control siRNA (non-targeting siRNA pool) or PHD1/PHD2/PHD3 siRNA targeting siRNA pool as indicated. Cells were maintained for 48h after transfection before being harvested for mRNA quantitation (A) or determination of HRE-luciferase activity (B). PHD (1–3) mRNA levels were quantitated by real-time PCR. p<0.05, *, lower (A) or higher (B) compared to the corresponding group treated with control siRNA. Results are mean ± S.D.