FIG. 2.

TbMT417 modifies the second position of SL RNA. A. RNA was collected at 2 days from pZJMTbMT417 cells grown in the presence (+) and absence (−) of Tet. The RNA was run on a 1.1% formaldehyde agarose gel, blotted, and probed for the presence of TbMT417 mRNA. The ethidium bromide stain of the rRNAs is a loading control. B. Primer extension analysis using total RNA collected from pJZMTbMT417 RNAi inductions at days 2, 4, and 5. Extension products from the TbSL40 primer were separated on a 6% acrylamide-8 M urea gel and run next to a cognate sequence ladder.