FIG. 3.

TbMT511 removal leads to cap 2 accumulation. A. RNA was collected after 4 days from pZJMTbMT511 cells grown in the presence (+) and absence (−) of Tet. The RNA was run on a 1.1% formaldehyde agarose gel, blotted, and probed for the presence of TbMT511 mRNA. The ethidium bromide stain of the rRNAs is a loading control. B. Primer extension analysis using total cell RNA collected from clonal pJZMTbMT511 RNAi cell lines at days 1 and 4 after Tet induction. Extension products were separated on a 6% acrylamide-8 M urea gel and run next to the cognate sequence ladder.