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. 2006 Jun;5(6):905–915. doi: 10.1128/EC.00080-06

FIG. 5.

FIG. 5.

Undermethylated SLs are trans-spliced onto mRNA. In the left panels, poly(A)+-selected mRNA was extended using TbWTexon, complementary to the exon sequence, and separated on a 6% acrylamide-8 M urea gel and run next to the cognate sequence ladder. In the right panels poly(A)+-selected mRNA was extended with TbSL40 to control against contamination by substrate SL RNA. A. Induced (+) and uninduced (−) mRNAs from pZJMTbMT417 cells. B. Induced and uninduced mRNAs from pZJMTbMT511 cells. C. Induced and uninduced mRNAs from pZJMTbMT417-TbMT511 cells.