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The PCR reaction products obtained using a 17-bp primer corresponding to bases 1,171 to 1,188 of the open reading frame of mI 1-PS-1b and a second, reverse strand primer where the 1,171 to 1,188 primer ends either in G (wild-type sequence) or T (LR33 sequence). The reaction was run with an annealing temperature of 62°C. Both primers give roughly equal reaction products in lines that are heterozygous for the LR33 mutation.
