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. 2002 Feb;128(2):650–660. doi: 10.1104/pp.010585

Figure 5.

Figure 5

A photograph of the Coomassie Blue-stained SDS-PAGE protein gel of soluble proteins from E. coli after cell disruption and ammonium sulfate fractionation. Lane 1, Mr markers; lane 2, extract from cells expressing wild-type GM-mI-PS-1a under control of the T7 promoter; lane 3, extract from wild-type GM-mI-PS-1a in which residue K396 was changed to N396 by site directed mutation; lane 4, GM-mI-PS-1a from the LR33 mutant; lane 5, an extract from control E. coli DE3 cells. The specific activity of myo-inositol 1-phosphate synthase in lanes 2 through 4 is listed below the lane.