FIG. 2.
HPLC analysis of the A. fumigatus strains used in this study. Chloroform extracts of CFs from Czapek Dox broth cultures grown for 72 h at 37°C. Gliotoxin and unknown metabolite peaks are identified. Panels: A, gliotoxin standard, 1 mg/ml; B, wild-type AF293; C, ectopic transformant ARC5; D, ΔgliP mutant strain ARC2 (lacks the peak at approximately 8.3 min, which demonstrates elimination of gliotoxin production in this mutant).