TABLE 3.

Analysis of gliotoxin biosynthesis cluster gene expression in ΔgliP mutant strain ARC2^a

Gene	Product	ARC2	ARC2 + 500 ng/ml gliotoxin	ARC2 + 5 μg/ml gliotoxin	ARC2 + 20 μg/ml gliotoxin	Wild type + 20 μg/ml gliotoxin
gliA	MFS transporter	0.00 ± 0.00	0.01 ± 0.00	0.33 ± 0.05	2.10 ± 0.27	1.37 ± 0.69
gliC	Cytochrome P450 monooxygenase	0.29 ± 0.01	0.57 ± 0.05	0.79 ± 0.07	1.29 ± 0.41	1.04 ± 0.49
gliF	Unknown	0.11 ± 0.01	0.55 ± 0.10	0.39 ± 0.07	2.30 ± 0.52	1.28 ± 0.63
gliG	Glutathione S-transferase	0.04 ± 0.01	0.05 ± 0.15	0.10 ± 0.3	3.18 ± 0.62	1.01 ± 0.50
gliI	Aminocyclopropane carboxylate synthase	0.30 ± 0.04	2.96 ± 0.24	1.02 ± 0.15	2.70 ± 1.10	1.22 ± 0.63
gliJ	Dipeptidase	0.45 ± 0.02	1.23 ± 0.27	0.66 ± 0.09	1.18 ± 0.84	2.12 ± 1.20
gliK	Unknown	0.09 ± 0.03	0.34 ± 0.03	0.15 ± 0.02	3.43 ± 0.51	1.57 ± 0.75
gliM	O-Methyltransferase	0.03 ± 0.00	0.27 ± 0.09	0.19 ± 0.05	4.81 ± 0.72	1.22 ± 0.63
gliN	Methyltransferase	0.16 ± 0.00	1.00 ± 0.15	0.65 ± 0.14	2.70 ± 1.28	1.85 ± 0.88
gliP	Peptide synthetase	0.00 ± 0.00	0.00 ± 0.00	0.00 ± 0.00	0.00 ± 0.00	1.88 ± 0.91
gliT	Thioredoxin reductase	0.09 ± 0.00	0.85 ± 0.47	0.32 ± 0.21	2.7 ± 1.34	0.75 ± 0.61
gliZ	Zinc finger transcription factor	0.03 ± 0.00	0.12 ± 0.02	0.14 ± 0.06	4.29 ± 0.24	1.82 ± 1.18

^aThe wild-type strain and ΔgliP mutant strain ARC2 were incubated in Czapek Dox broth for 24 h, when various concentrations of gliotoxin (0, 500 ng/ml, 5 μg/ml, and 20 μg/ml) were added and the cultures were incubated for an additional 24 h before total RNA extraction. Total RNA (500 ng) was reverse transcribed and used in a real-time RT-PCR. Data are presented as the mean fold change in expression (2^−ΔΔCt) from wild-type AF293 (fold change = 1) ± the standard deviation. Expression of each gene under each condition is normalized to that of the gene for actin. Addition of 20 μg/ml gliotoxin restored expression of the gliotoxin biosynthesis gene cluster in ΔgliP mutant strain ARC2 but did not repress expression when added to the wild type.