FIG.1.

Identification of EppA by 2-D electrophoresis of ³²P-labeled cells. (A) Coomassie brilliant blue-stained 2-D gels of wild-type and Dderk2⁻ cells before and 1 min after stimulation with cAMP. The box in the wild-type 1-min sample is shown at higher magnification in panel C. Black squares in diagonally opposite corners are filter papers containing ³²P for registration of the autoradiograms in panel B. (B) Autoradiography of 2-D gels in panel A. Phosphoproteins present in three experiments and quantitated in Table 1 are labeled in the autoradiogram of the stimulated wild-type sample. (C) DdERK2-dependent protein position shift and phosphorylation change of EppA. The arrowhead points to the position of phosphorylated EppA (spot 3 in Fig. 1B), and arrows indicate the corresponding position in samples from unstimulated or DdErk2⁻ cells.