TABLE 3.
Quantitation of cAMP chemotaxisa
| Strain (n)b | Avg cosθc | Avg velocity (μm/min)c | Persistencec |
|---|---|---|---|
| wt/pBsr (853) | 0.49 ± 0.01 | 6.31 ± 0.39 | 0.64 ± 0.03 |
| eppA− (424) | 0.29 ± 0.04** | 4.46 ± 0.35* | 0.55 ± 0.01* |
| eppA−/myc-EppA (941) | 0.56 ± 0.02 | 6.67 ± 0.26 | 0.69 ± 0.03 |
| wt (638) | 0.58 ± 0.04 | 6.41 ± 0.71 | 0.73 ± 0.05 |
| wt/vector (602) | 0.62 ± 0.02 | 6.17 ± 0.75 | 0.75 ± 0.03 |
| wt/myc-EppA (925) | 0.58 ± 0.01 | 6.29 ± 0.16 | 0.75 ± 0.01 |
| wt/myc-EppA(S250A) (506) | 0.36 ± 0.06* | 5.75 ± 0.48 | 0.61 ± 0.02 |
a
Cell chemotaxis was measured on 8-h-starved cells in a Zigmond chamber with a 2 μM cAMP gradient.
b
wt, wild type; n, total number of cells analyzed in three experiments.
c
Data are means ± standard errors from three experiments. *, P < 0.05; **, P < 0.01.