FIG. 3.

Vacuoledynamics and interconnection in hyphae of P. velutina using time-lapse 4D confocal imaging. (A) Maximum projections cropped from every 12th image of a 4D (984 by 143 by 8 by 67 pixels) sequence at the times (s) indicated. A series of large vacuoles are appressed to the plasma membrane but remain connected by a highly dynamic set of longitudinal tubules, often with more than one tube (arrows) connecting each vacuole (see Video S3A in the supplemental material). Bar = 10 μm. (B) Movement of a detached large vacuole (arrows), visualized as maximum projections cropped from a 4D (512 by 512 by 11 by 12 pixels) sequence lasting 60 min at the times (s) indicated. Bar = 10 μm.