TABLE 1.
Oligonucleotides used in this study
| Primer | Orientation | Sequence (5′ to 3′) | Fragment size (bp)a |
|---|---|---|---|
| 1B | Forward | TCTCCCGAACGTCTTCAAGCCA | |
| 1C | Reverse | ATCATTCCTCCACATAACAAAGG | 310 |
| 2C | Reverse | GCTTCCACACATGTTCACATTC | 318b |
| 3A | Forward | AGACGACGTGACGGTAATGGG | |
| 3B | Reverse | AACATCGCCACTCTCATCGGAT | 280 |
| 4A | Forward | ACTATGCTCTACAGAAGGAGCTGAA | |
| 4B | Reverse | ACTTCCAACCACCTTGTATGTCAC | 422 (618) |
| 6A | Forward | AGATGGGCTTTCTTTCCTCTTGG | |
| 6B | Reverse | CTATACAACCGCGTAAACAAATG | 148 |
| 7A | Forward | ACAAGGCACGCCATCCGCCG | |
| 7B | Reverse | TCGGTACTACTCAGTCGCGTC | 291 |
| 8A | Forward | TTGCTGTTATCTCTGCTACTGCG | |
| 8B | Reverse | TACCCTATTCCTCCTGGTTTAGG | 280 |
| 9A | Forward | TGGAGAGTTTCCTCCGGCGGT | |
| 9B | Reverse | AAGGCTGTATAAAGTCGCAAACAT | 278 |
| 10A | Forward | CAAGAGTTTGAGACATCATGGTGA | |
| 10B | Reverse | AACCATATGTCTCTACGGAAGGC | 280 |
| 11A | Reverse | ATCATAATCATCATCTTCTAAACTG | |
| 11B | Forward | ATGACAAAGAGCGAAGAGAAGG | 285 |
| 12A | ? | GTTGAAGGCGTGTGCCATAGG | |
| 12B | ? | ACAACCACACTTCGACGAGTAC | 119 |
| 13A | Reverse? | AGCGTTCTGTTGTAGGTCGAAG | |
| 13B | Forward? | GGGAATATTAACCCGTTGTCCAT | 207 |
| 14A | Forward | ACACACCAAGGCATCAACAAGC | |
| 14B | Reverse | GGCCTTATTGGAGAACGAGACA | 261 |
| 15A | Forward | CAACAATGTGCGTGCTCAGTATC | |
| 15D | Reverse | ACAGCACGGTTGAATCCGTAGT | 171 (283) |
| 16A | Reverse | TTGCTACCAGCACTCCTCCTCT | |
| 16B | Forward | ACGTCTCCGCATTGGATCGCA | 309 |
| 17A | Forward | GAGTGGAAAGGCCGTCACTCAA | |
| 17B | Reverse | ACTACCGCCCCAGGATGCGAT | 129 |
| 18B | Reverse | GCATGCATCTCCGTCGGGATC | |
| 18A | Forward | ACTGTCCAAATTGCGGAGGTGG | 322 |
| 19A | Forward | TCGCCTGATCAAGGCTCAAGGA | 277c |
| p150A | Forward | TTCAACGCATCTACTACAAG | |
| p150B | Reverse | GAGTGCAGCAGTGATGTAGAGC | 334 |
| p150-likeA | Forward | TCCTGTGTGCGACGACCTTTCA | |
| p150-likeB | Reverse | ACTTCGTGGTACTCCCTCAACAT | 306 |
| NR1 | Forward | CCTGGATACATTGGTGGAAGGAT | |
| NR2 | Reverse | GGAGGCAAGATACGATTATCATG | 106 |
| GPX1 F | Forward | CAAAGGCGACGTGCTATGCGTC | |
| GPX1 R | Reverse | GGCTCCTGAGCTCCAAACTGATT | 192 |
| GPX2 F | Forward | TTCGGAGCAACTTTCCAGAGGTG | |
| GPX2 R | Reverse | TGCCCATAACTCTTGACAGCCCT | 166 |
| GPX-like F | Forward | AAACTCCACCGCAAATACAAATCC | |
| GPX-like R | Reverse | CACGTAATCCTAGGAGGACCG | 229 |
| Catalase F | Forward | GTTGATGATTCGGTTGGCTTGGC | |
| Catalase R | Reverse | AGTTGAGAGGTGCAAGACGGATG | 101 (195) |
a
Genomic fragment sizes are in parentheses in cases in which the primers encompass an intron.
b
Cue2 was amplified by primers 1B and 2C.
c
Cue19 was amplified by primers 18B and 19A.