TABLE 2.
RT-PCR results
| Gene(s) | No. of clones sequenced from subtracted cDNA library | Cu-induced fold change in mRNA concn
|
H2O2-induced fold change in mRNA concn
|
||
|---|---|---|---|---|---|
| 1 h | 24 h | 1 h | 24 h | ||
| Potential Cu-expressed genesa | |||||
| 1 | 11 | 1,977 | 4 | 2.9 | 5.1 |
| 2 | 11 | 1,545 | 23 | 2 | 8.4 |
| 3 | 1 | 49 | 21 | 3.4 | 19.9 |
| 4, 5 | 1 | 1 | 2 | 0.4 | 5 |
| 6 | 14 | 1,826 | 11 | 2 | 0.9 |
| 7 | 14 | 1,249 | 7 | 1.1 | 0.7 |
| 8 | 1 | 46 | 5 | 1.5 | 1.1 |
| 9 | 1 | 4 | 1 | 2.7 | 2.1 |
| 10 | 1 | 7 | 2 | 3.7 | 3.2 |
| 11 | 2 | 1,101 | 203 | 2 | 2.3 |
| 12 | 1 | 8 | 3 | 1.4 | 2.2 |
| 13 | 1 | 0.4 | 0.4 | — | — |
| 14 | 1 | 2 | 2 | 1.8 | 2.6 |
| 15 | 2 | 13 | 2 | 1.5 | 5.1 |
| 16 | 1 | 0.9 | 0.1 | — | — |
| 17 | 1 | 0.6 | 0.8 | — | — |
| 18 | 0 | 514 | 16 | 0.8 | 4.6 |
| 19 | 0 | 772 | 25 | 0.8 | 11 |
| Cu-expressed genesb | |||||
| p150 | 0 | 2 | 26 | 1.3 | 4.7 |
| p150-like | 0 | 1 | 2 | 2 | 1.4 |
| Genes potentially involved in H2O2 degradation | |||||
| GPX1 | 0 | 0.8 | 0.3 | 1.4 | 0.7 |
| GPX2 | 0 | 1.5 | 1.1 | 1.3 | 1.6 |
| GPX-like | 0 | 2.7 | 2.3 | 2.1 | 2.2 |
| Catalase | 0 | 2 | 2.4 | 0.5 | 0.6 |
a
Details of 1-h forward subtracted cDNA library and RT-PCR results. —, not determined.
b
RT-PCR results for genes previously shown to be induced by 24 h of Cu exposure.