FIG. 3.

Overexpression of OYE2 or OYE3 increases resistance to acrolein. (A) Wild-type strains containing empty vector pYES/CT (v) or vector-containing OYE2 (mcOYE2) or OYE3 (mcOYE3) were grown to exponential phase in minimal SD media. Cells were washed and resuspended in minimal media containing glucose or galactose (Gal) to induce the expression of OYE2 and OYE3. Cultures were adjusted to A₆₀₀ values of 1, 0.1, and 0.01 before being spotted onto plates. Results are shown for plates containing no oxidant (YEPD), 4 mM acrolein, 4 mM H₂O₂, and 2.5 mM crotonaldehyde (Crot). Chemical structures are shown for acrolein (Acr) and crotonaldehyde. (B) Overexpression of OYE2 and OYE3 was confirmed by means of Western blot analysis.