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Regulation of OYE2 and OYE3 expression in response to acrolein. Gene expression of OYE2 (A) and OYE3 (B) was determined by RT-PCR. Wild-type (wt), yap1, skn7, and msn2 msn4 mutant cells were grown to exponential phase and treated with 0.05 mM or 0.1 mM acrolein for 1 h. Induction (n-fold) is expressed relative to ACT1 and relative to the untreated sample for each strain. The values shown are the means for three determinations.
