Figure 4.

Effect of KICA on DNA fragmentation of in rat glioma cells in culture. (A) Cultures were incubated for 20 h with DMEM/0.5% FCS containing 25 mM KICA, and the morphology was investigated by light microscopy after ISEL. Healthy and apoptotic cells are indicated by arrowheads and arrows, respectively. Magnification, 175×. (B) Analysis of nucleosome laddering in C6 rat glioma cells at defined time points after treatment with DMEM/0.5% FCS containing 50 mM KICA. DNA molecular weight markers are indicated (in base pairs) by the arrows.