Table I.
Accumulation of major benzophenanthridine alkaloids in cell suspension cultures of E. californica transformed with 35S∷GUS (control-1 and control-2), 35S∷antiBBE (BBE-A1 and BBE-A2), and 35S∷antiCYP80B1 (CYP80B1-A1 and CYP80B1-A2)
| Alkaloid | Cell Line
|
|||||
|---|---|---|---|---|---|---|
| Control-1 | Control-2 | BBE-A1 | BBE-A2 | CYP80B1-A1 | CYP80B1-A2 | |
| nmol g−1 (dry wt) | ||||||
| Macarpinea | 40.7 ± 47.1 | 14.5 ± 10.6 | Trace | 2.4 ± 2.7 | 7.2 ± 11.7 | 24.8 ± 16.5 |
| Dihydromacarpine | 278.9 ± 38.2 | 224.4 ± 128.5 | 55.3 ± 13.2 | 297.1 ± 175.7 | 76.3 ± 92.1 | 246.1 ± 141.4 |
| Chelerythrine | 3.7 ± 4.5 | 0.7 ± 0.7 | Trace | 2.4 ± 2.7 | 4.3 ± 4.0 | 0.7 ± 0.7 |
| Dihydrochelerythrine | 8.3 ± 0.7 | 8.0 ± 4.1 | Trace | Trace | 10.1 ± 5.5 | 10.0 ± 9.5 |
| Chelilutine/chelirubine | 28.4 ± 27.9 | 11.3 ± 8.3 | 2.8 ± 2.8 | 7.1 ± 7.8 | 3.7 ± 2.0 | 12.1 ± 9.8 |
| Dihydrochelilutine | 10.9 ± 4.1 | 7.0 ± 3.7 | 26.8 ± 6.8 | 109.7 ± 73.7 | 73.8 ± 75.8 | 193.7 ± 99.7 |
| Sanguinarine | 1.1 ± 1.5 | 0.4 ± 0.2 | Trace | 1.3 ± 0.7 | 2.1 ± 2.1 | 6.0 ± 9.9 |
| Dihydrosanguinarine | 10.9 ± 3.6 | 9.7 ± 7.4 | Trace | Trace | 4.4 ± 3.9 | 22.1 ± 38.3 |
| m/z = 338b | 484.4 ± 132.4 | 413.7 ± 245.8 | 0.6 ± 0.2 | 113.8 ± 161.4 | 57.1 ± 36.4 | 127.7 ± 38.0 |
| Totalc | 868.7 ± 248.6 | 690.0 ± 408.3 | 89.5 ± 21.1 | 544.5 ± 377.8 | 250.6 ± 207.6 | 627.2 ± 209.4 |
a
Values represent the mean ± sd of three independent experiments.
b
Unknown alkaloid with a Mr of 338.
c
Represents the sum of all major alkaloids.