FIG. 6.

Transcription profiles of purine and riboflavin genes in wild-type and Δbas1 strains during the trophic and productive phases. Transcription profiling of the wild-type (WT) and Δbas1 strains grown in MA2 rich medium was achieved at different time points. In the wild-type strain (upper panel), the transcription of ADE4 and SHM2 decreased along the growth curve, whereas the transcription of RIB1 and RIB3 increased during the productive phase. In the Δbas1 strain (lower panel), ADE4 and SHM2 show a constitutive deregulated transcription. Transcription levels are normalized with the transcription rate of AgACT1.Data are representative of four experiments with similar results and are presented as log₂ of the 2^−ΔΔCt (ΔΔCt = ΔCt_T − ΔCt_{12 h}). T, incubation time (h).