FIG. 7.

Characterization of the ΔC631BAS1 strain. (A) Growth pattern of ΔC631BAS1 strain grown in liquid MA2 rich medium in comparison with the wild-type and Δbas1 strains. (B) Relative transcription levels of purine and riboflavin genes in the wild-type (gray bars) and the ΔC631BAS1 (white bars) strains grown in MA2 rich medium with (+ade) or without (−ade) an excess of adenine (0.1 g/liter). Transcription levels are normalized to the level of AgACT1 mRNA. The ratio of the relative transcription of the target gene was calculated as 2^−ΔΔCt, where ΔΔCt = ΔCt_{MA2 + ade} − ΔCt_{MA2 − ade}. An average of three separate cDNA dilutions from each target gene was obtained, and the relative transcription levels are expressed as log₂ ± SD.