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Phage B30 endolysin (443-amino-acid) conserved domains and deletion constructs. The schematic diagram at the top indicates the locations of the NH2-terminal CHAP endopeptidase, Acm glycosidase, C-terminal SH3b cell wall binding domain, and the six-His tag (solid box) in the pET21a-derived construct. The numbers indicate the initial and final amino acids in the deletion constructs and the C-terminal six-His tag.
