FIG. 3.

ABL-1 mutant nematodes are resistant to S. flexneri-mediated C. elegans killing. (A) Young adult hermaphrodite abl-1(ok171) nematodes were fed on lawns of either E. coli OP50, S. flexneri 2457T, or S. enterica serovar Typhimurium SL1344 at 25°C, and nematodes were scored daily for survival. The survival curve represents data from 5 independent experiments, each using 20 nematodes. These experiments also included analysis of N2 nematodes, shown in Fig. 1. (B) The time required for nematodes to die (TD₅₀) was calculated from the survival data in panel A, representing data from three independent experiments. The asterisk indicates significant difference compared to results for OP50 (P < 0.05). (C) The relative mortality was calculated using the TD₅₀ values for N2 and abl-1(ok171) nematodes in order to normalize the survival rates of the nematodes grown on pathogens compared to that of nematodes fed on E. coli OP50 (2).