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. 2006 Jul;72(7):4717–4725. doi: 10.1128/AEM.00492-06

FIG. 2.

FIG. 2.

Test of the regulatory function of the insertion element InsTetG−1 cloned in front of a promoterless lacZ gene and cotransformed with TetR-expressing plasmids. The large regulatory window of InsTetG−1 was determined by measurements of β-gal activity. The percent β-gal activity can be seen on the y axis. The left panel shows the results for E. coli and the right panel shows results for Salmonella serovar Typhimurium with atc (black columns) and without atc (white columns). Measurements without a regulator (−) represent the 100% values, with 1,120 Miller units (MU) (with atc) and 1,200 MU (without atc) for E. coli and 2,350 MU (with atc) and 1,720 MU (without atc) for Salmonella serovar Typhimurium. TetR and revTetRr2 represent the two TetR variants expressed from pWH1411.