TABLE 1.
Bacterial strains and plasmids used
| Strain or plasmid | Genotype | Reference or source |
|---|---|---|
| Strains | ||
| E. coli K-12 | ||
| DH5α | recA1 endA1 gyrA96 thi relA1 hsdR17 (rK− mK+) supE44 φ80dlacZΔ ΔlacU169 | 4 |
| MG1655 | rph-1 | 5 |
| ER2566 | fhuA2 lacZ::T7 gene 1 [lon] ompT gal sulA11 R(mcr-73::mini-Tn10-Tets)2 [dcm] R(zgb-210::Tn10-Tets) endA1Δ (mcrC-mrr) 114::IS10 | 7 |
| Salmonella enterica serovar | Wild type | ATCC 14028 |
| Typhimurium NCTC12023 | ||
| Plasmids | ||
| pCB302b | Apr, pBR322 derivative, divergent promoterless lacZ and galK | 29 |
| pDG972 | Kmr, pMTL23 derivative | 14 |
| pTYB2 | Cmr, pACYC derivative, C-terminal intein-chitin binding domain fusion | 7 |
| pUC18 | AprlacZα | 37 |
| pWH1411BD | Cmr, pACYC177 derivative, tetR(BD)51-208 | 31 |
| pWH1411r2 | Cmr, pACYC177 derivative, tetRr2(BD)E15A, L17G, L25V; XbaI/NcoI from pWH1925-TetRr2 | This study |
| pWH1865 | Apr/Kmr, pUC18 derivative, InsTetG−1 in XbaI/NcoI pWH1865 ΔKm | This study |
| pWH1865ΔKmr | Apr, pUC18 derivative, InsTetG−1 ΔaphAIII XbaI/NcoI | This study |
| pWH1866 | Apr/Kmr, pUC18 derivative, InsTetG−1 flanked by PvuII sites | This study |
| pWH1867 | Apr/Kmr, pCB302b derivative, InsTetG−1 upstream of a promoterless lacZ | This study |
| pWH1891 | Apr, pTYB2 derivative, Tn5 transposase E54K, M56A, L372P; C-terminal intein-chitin binding domain fusion | This study |
| pWH1925r2 | Apr, pUC19 derivative, tetR(BD)E15A, L17G, L25V | 30 |