TABLE 2.
Insertion mutants of E. coli and functions of the affected genesa
| Classification | Gene(s) | Pathway |
|---|---|---|
| Biosynthetic pathways | argC, D, E, G | Arginine biosynthesis |
| aroB | Chorismate biosynthesis | |
| bioA, B | Biotin biosynthesis | |
| carB | Pyrimidine and arginine biosynthesis | |
| cvpA | Colicin V biosynthesis | |
| cysD, G, J, Q | Sulfur metabolism | |
| entB, F | Enterobactin biosynthesis | |
| hflD | Membrane protein in operon with purB | |
| hisB | Histidine biosynthesis | |
| ilvA, C, D | Valine, isoleucine biosynthesis | |
| leuA, B, C | Leucine biosynthesis | |
| menD | Menaquinone biosynthesis | |
| metA, B, C, E | Methionine biosynthesis | |
| panB, C | Pantothenate biosynthesis | |
| pdxB | Pyridoxal 5′-phosphate biosynthesis | |
| proA | Proline biosynthesis | |
| purC, H | Purine nucleotide biosynthesis | |
| pyrD, F | Pyrimidine ribonucleotide biosynthesis | |
| rfaG | Lipopolysaccharide core biosynthesis | |
| serA, C | Serine biosynthesis | |
| thiC | Thiamine biosynthesis | |
| thrA | Homoserine biosynthesis | |
| trpA, B, C, E | Tryptophan biosynthesis | |
| tyrA | Phenylalanine/tyrosine biosynthesis | |
| Catabolism | fruK | 1-Phosphofructokinase |
| gltA | Citrate synthase | |
| mtlA | Subunit of EIImtlA | |
| pgi | Phosphoglucose isomerase | |
| ppc | Phosphoenolpyruvate carboxylase | |
| Regulation | argP | Transcriptional regulator |
| cspA | Transcriptional activator | |
| cysB | Transcriptional regulator | |
| ilvY | Transcriptional regulator | |
| Transporters | nirC | Nitrite transporter |
| cysP | Thiosulfate ABC transporter | |
| glcA | Glycolate transporter | |
| xylE | Xylose major facilitator superfamily transporter | |
| Essential genes | lhr | Member of ATP-dependent helicase superfamily II |
| mraZ | Member of dcw cluster (cell division) | |
| prmA | Methylation of 50S ribosomal subunit protein L11 | |
| rplM | L13 protein of 50S ribosomal subunit | |
| sfmH | Involved in fimbrial assembly | |
| truA | Pseudouridylate synthase I | |
| Unknown function | b3557 | IS5-related protein |
| ydeO | Putative AraC-type regulatory protein | |
| yfgL | Putative dehydrogenase | |
| yhdP | Putative transport protein | |
| yidL | Putative AraC-type regulatory protein | |
| yjhF | Member of Gnt transporter family | |
| ykgC | Putative oxidoreductase | |
| yrbK | Conserved hypothetical protein |
a
Genes are classified depending on their function; regulatable candidates are shown in boldface type.