TABLE 3.

All regulatable auxotrophic E. coli mutants^a

Designation	Gene	Function	Phenotype	Regulator
A	aroB	3-Dehydroquinate synthase	regaux	revTetRr2
B1	b3557	IS5 protein	revreg	TetR
B2	b3557	IS5 protein	revreg	TetR
B3	b3557	IS5 protein	revreg	TetR
B4	cspA	Intergenic region downstream of cspA ccw	revreg	TetR
C	cysP	Thiosulfate ABC transporter	regaux	TetR
D	cysQ	Involved in sulfite synthesis	regaux	TetR
E	ilvY	Transcriptional dual regulator	regaux	revTetRr2
F	mraZ	Involved in cell wall synthesis and cell division	regaux	revTetRr2
G	nirC	Nitrite FNT transporter	regaux	TetR
H	pyrF	Subunit of orotidine-5′-phosphate-decarboxylase	regaux	revTetRr2
I	rplM	Upstream of rplM (product, 50S ribosomal subunit protein L13)	regaux	revTetRr2
K	thiC	Thiamine biosynthesis	regaux	TetR
L	trpE/trpL	Subunit of anthranilate synthase	regaux	revTetRr2
M	yrbK	Conserved hypothetical protein	regaux	revTetRr2

^aThe involved genes are depicted with their functions. “regaux” indicates the regulatable auxotrophic phenotype, whereas “revreg” denotes the reverse regulatable phenotype. “revTetR^r2” denotes the reverse TetR^r2 variant present in the respective strains.