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Summary of the purification of the crude ABA-binding proteins from the lower epidermis of bean leaves
| Purification Step | Protein | Bmax | Kd | ABA-Binding Activity | Yield | Purification |
|---|---|---|---|---|---|---|
| mg | nmol g−1 protein | nm | nmol | % | -fold | |
| Crude extra | 2,531.2 | 68 | 19 | 172 | 100 | 1 |
| Affinity | 1.17 | 20,700 | 21 | 24 | 14 | 304 |
| Chromatography |
Bmax, Maximum ABA-binding activity; Kd, equilibrium dissociation constant.
