FIG. 4.

Western blot analysis (A, C, and D) of P. aeruginosa PAO1 proteins after SDS-PAGE for biotin-containing proteins. Soluble cell extracts after avidin purification were separated on 10% SDS-polyacrylamide gels and stained with Coomassie brilliant blue (B) or transferred to polyvinylidene difluoride membranes and screened for biotin-containing proteins (A and C). Gene products identified by trypsin peptide analysis of isolated protein bands from panel B are indicated. Western blot analysis of P. aeruginosa PAO1 ins-atuR proteins after SDS-PAGE for biotin-containing proteins (D). Note the constitutive expression of AtuF. PAO1-WT, PAO1 (wild type); ins-liuD, insertion liuD mutant; ins-atuR, insertion atuR mutant.