TABLE 3.

Proteins, genes, and assigned functions in catabolism of acyclic terpenes and isovalerate^a

Spot no.	PA no.	Proposed name	No. of peptides that matched	% Coverage	Expression of proteins in cells grown onb:				Proposed function
					Succ	IsoV	CL	CA
1	2886	AtuA	12	32	−	−	+	+	3-Hydroxy-3-isohexenylglutaryl-CoA:acetate-lyase?
2	2887	AtuB	6	32	−	−	+	+	Citronellol/citronellal dehydrogenase
3	2888	AtuC	WB		−	−	+	+	GCase, β-subunit
4	2889	AtuD	ND						Citronellyl-CoA dehydrogenase
5	2890	AtuE	8	34	−	−	+	+	Isohexenylglutaconyl-CoA hydratase
6	2891	AtuF	20/WB	44	−	−	+	+	GCase, α-subunit (biotin containing)
7	2892	AtuG	20	68	−	−	+	+	Citronellol/citronellal dehydrogenase
8	2893	AtuH	ND						Citronellyl-CoA synthetase
9	1342		15	42	−	−	+	+	Periplasmic binding protein (ABC transporter)
10	2015	LiuA	15	37	−	+	+	+	Isovaleryl-CoA Dehydrogenase
11	2014	LiuB	19/WB	45	−	+	+	+	MCase, β-subunit
12	2013	LiuC	9	28	−	+	+	+	Methylglutaconyl-CoA hydratase
13	2012	LiuD	WB		−	+	+	+	MCase, α-subunit (biotin containing)
14	2011	LiuE	ND						3-Hydroxymethylglutaryl-CoA lyase
15	1999	AtoD	6	41	−	+	+	+	Acetyl-CoA:acetoacetate-CoA transferase
16	2001	AtoB	15	75	±	+	+	+	Acetyl-CoA acetyltransferase
17	2634	Icl	16	33	±	+	+	+	Isocitrate lyase
18	5035	GltD	16	40	±	±	±	±	Glutamate synthase

^aHypothetical proteins are given in normal roman type. Proteins with experimentally verified function are given in bold type. Expression of proteins was shown in cells grown on succinate (Succ), isovalerte (IsoV), citronellol (CL), and citronellate (CA) by 2D gel electrophoresis or by Western blotting (WB) and trypsin peptide analysis or not determined (ND).

^bSymbols: −, no expression; +, significant expression; ±, poor or uncertain expression.