TABLE 1.
Ratios of relative fluorescence units resulting from LH-PCR products following 15 and 20 cycles of PCR in which unlabeled F. pelagi and BrdU-labeled B. vulgatus DNA templates at known ratios were amplified using primers 27F and 338R
F. pelagi/B. vulgatus ratio in template |
F. pelagi/B. vulgatus ratio in PCR products
|
|
---|---|---|
15 cycles | 20 cycles | |
1:10 | 0.0597 | 0.2540 |
1:1 | 0.5660 | 0.7162 |
10:1 | 5.060 | 6.261 |