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. 2006 Jul;72(7):4532–4539. doi: 10.1128/AEM.00038-06

TABLE 1.

Ratios of relative fluorescence units resulting from LH-PCR products following 15 and 20 cycles of PCR in which unlabeled F. pelagi and BrdU-labeled B. vulgatus DNA templates at known ratios were amplified using primers 27F and 338R

F. pelagi/B. vulgatus ratio in template F. pelagi/B. vulgatus ratio in PCR products
15 cycles 20 cycles
1:10 0.0597 0.2540
1:1 0.5660 0.7162
10:1 5.060 6.261