TABLE 2.

Fluorescent PCR efficiency with 15 cycles when known quantities of BrdU-labeled target DNA were immunocaptured in a community of BrdU-labeled DNA

Starting proportion (ng)		Detection of B. vulgatus before immunocapture			Detection of B. vulgatus following immunocapture
E. coli	B. vulgatus	Replicate 1	Replicate 2	Replicate 3	Replicate 1	Replicate 2	Replicate 3
0.99	0.01	+a	+	±	−	−	+
0.9	0.1	+	+	+	−	+	+
0.5	0.5	+	+	+	+	+	+
0.1	0.9	+	+	+	+	+	+
0.01	0.99	+	+	+	+	+	+

^a+, positive; −, negative; ±, weak positive (RFU values less than values that have statistical significance).