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Identification of antigen P18 of F. psychrophilum G4 by Western blotting. Samples were whole cell proteins (20 μg; lanes 1, 2, and 3) and HPLC-purified P18 (5 μg; lane 4) solubilized with 1% SDS. Protein bands were silver stained (lane 1) or probed with rabbit serum directed against HPLC-purified P18 (lane 2) or with a serum collected from a CWD-convalescent trout (lanes 3 and 4).
