FIG. 6.

Cellulose production by WT Rv and LT2 and their derivatives. Greater amounts of cellulose were produced by both the ddhC::Tn5 (A1-8) and waaG::Tn5 (A1-9) mutants than by WT Rv. Greater cellulose production was also obtained from the waaG deletion mutant YA155 than from WT LT2 and waaG-complemented strain YA156. Cellulose was isolated from 200 mg of lyophilized cell mass obtained from colonies of each strain streaked with a high-density inoculum on LB plates, followed by a 4-day incubation at 28°C. Control strains YA151 (ΔcsgA::kan) and YA159 (ΔbcsA::kan) were included as positive and negative controls, respectively. Error bars represent the standard deviations.