TABLE 2.

Primers used in this study

Primer	Sequence (5′ to 3′)	Source or referencec
pwaaG11	CCC AAG CTT CGA CGA CAT CAT TCA GTT Ta	5848-5830d
pwaaG12	CGG GGT ACC TAC CTT TAT GCC ACT TCA Ga	4401-4419d
pwaaGD11	GAA AAA ATG CTG CCG CAT GAG GCA CGC ACC ATA GAT TTG GAC AGC CTG CTG TGT AGG CTG GAG CTG CTT Cb	5681-5730d
pwaaGD12	TAG TGT GGT TAA CGG CGC TTT CAG CTC AAC CAT CTA AAT CAC CTG TAA TAC ATA TGA ATA TCC TCC TTA Gb	4531-4580d
pwaaGIN11	GAG GGC ATC AGG TTC GTG T	5547-5566d
pwaaGIN12	AGG CGT TGC GTA AGG AAG G	4643-4662d
pwaaPIN11	TTC CTG AAG TGG CAT AAA G	4387-4405d
pwaaPIN12	AAA TAC TCA CGC ATA AAC C	3872-3890d
pcsgAD11	ATG GCT ATT CGC GTC ACC CAA CGC TAA TAC CGT TAC GAC TTT TAA ATC AAG TGT AGG CTG GAG CTG CTT Cb	17701-17750e
pcsgAD12	TTT GAA AGT GCG GCA AGG AGC AAT AAA GTA TGC ATA ATT TCC TCC CGA AAC ATA TGA ATA TCC TCC TTA Gb	18271-18320e
pcsgA11	TCA CCC AAC GCT AAT ACC G	17713-17732e
pcsgA12	CCT TGC TGA GTC GTG GTA A	18332-18350e
pbcsAD11	ACG TCC GCC GGG AGC CTG CGA TGA GCG CCC TTT CCC GGT GGC TGC TTA TCG TGT AGG CTG GAG CTG CTT Cb	11930-11881f
pbcsAD12	TAT CAT CAT TGT TGA GCC TGA GCC ATA ACC CGA TCC GAC GGC TGT ATC GCC ATA TGA ATA TCC TCC TTA Gb	9281-9330f
pbcsA11	ATG ATG CGG GCG ACA AAA C	4937-4955f
pbcsA12	CCT ATT ACC GCC GCA CAC A	14221-14239f
p16s rRNA11	GAA GAG TTT GAT CAT GGC TC	21441-21460g
p16s rRNA12	TAC GGT TAC CTT GTT ACG AC	19953-19972g
pKanKD3	CAG TCA TAG CCG AAT AGC CT	Datsenko and Wanner (6)
inv2	GAA CTT TTG CTG AGT TGA AGG ATC A	Ducey and Dyer (9)

^aHindIII and KpnI sites are underlined.

^bSequence homologous to template plasmid pKD4 underlined.

^cSequence numbering from S. enterica serovar Typhimurium LT2.

^dNumbering from sequence with accession no. AF026386.

^eNumbering from sequence with accession no. AE008749.

^fNumbering from sequence with accession no. AJ315148.

^gNumbering from sequence with accession no. AE008820.