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Construction of recombinant plasmid pMMABCD. The arrows indicate the direction of transcription of the genes. Only relevant restriction sites are shown (EcoRI, XbaI, HindIII). Primers dszf, dszr, dszDf, and dszDr were used for amplification of dszABCD and dszD. bla, gene encoding ampicillin resistance; lacIq, gene encoding the LacI repressor; Ptac, promoter; MCS, multiple cloning site; SD, Shine-Dalgarno sequence; ori, sequence encoding the origin of replication for duplex DNA.
