TABLE 1.
Bacteria, phages, and plasmids
| Strain, bacteriophage, or plasmid | Relevant characteristicsa | Source or referenceb |
|---|---|---|
| Strains | ||
| E. coli | ||
| DH5α | F− (φ80dlacZΔM15) Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rK− mK+)supE44 λ−thi-1 gyrA relA | 24 |
| EC100D pir-116 | F−mcrA Δ(mrr-hsdRMS-mcrBC) φ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ−rpsL nupG pir-116 (DHFR) | Epicenter Technologies |
| Campylobacter sp. | ||
| T1 | Phage type strain 1, CRU reference no. C605 | CRU |
| T2 | Phage type strain 2, CRU reference no. C682 | CRU |
| T5 | Phage type strain 5, CRU reference no. C856 | CRU |
| T14 | Phage type strain 14, CRU reference no. C10054 | CRU |
| T33 | Phage type strain 33, CRU reference no. C1873 | CRU |
| T34 | Phage type strain 34, CRU reference no. C13503 | CRU |
| T36 | Phage type strain 36, CRU reference no. C1758 | CRU |
| T44 | Phage type strain 44, CRU reference no. C10131 | CRU |
| NCTC 11168 | Clinical isolate used for genome sequencing | 41 |
| NCTC 12658 | Propagating strain for Φ9 | CRU |
| NCTC 12659 | Propagating strain for Φ16 | CRU |
| NCTC 12660 | Propagating strain for Φ7 and Φ13 | CRU |
| NCTC 12661 | Propagating strain for Φ1 and Φ2 | CRU |
| NCTC 12662 | Propagating strain for Φ14 | CRU |
| NCTC 12663 | Propagating strain for Φ4 and Φ12 | CRU |
| NCTC 12664 | Propagating strain for Φ5 and Φ11 | CRU |
| NCTC 12665 | Propagating strain for Φ6 | CRU |
| NCTC 12666 | Propagating strain for Φ8 | CRU |
| NCTC 12667 | Propagating strain for Φ3 | CRU |
| NCTC 12668 | Propagating strain for Φ10 and Φ15 | CRU |
| 11168 motA::cat | C. jejuni NCTC 11168 with cat cassette in the motA gene; Cmr | This study |
| 11168 motB::cat | C. jejuni NCTC 11168 with cat cassette in the motB gene; Cmr | This study |
| 11168 ΔpflA::cat | C. jejuni NCTC 11168 in which pflA gene is deleted and replaced by a cat cassette; Cmr | This study |
| 11168 ΔflhA::cat | C. jejuni NCTC 11168 in which flhA gene is deleted and replaced by a cat cassette; Cmr | This study |
| 11168cj1428c | C. jejuni hypermotile 11168 with cj1428c::kan insertion; Kmr | - |
| 11168cj1429c | C. jejuni hypermotile 11168 with cj1429c::kan insertion; Kmr | - |
| 11168cj1439 | C. jejuni hypermotile 11168 with cj1439::kan insertion; Kmr | - |
| 11168kpsM | C. jejuni hypermotile 11168 with kpsM::kan insertion; Kmr | 30 |
| AG318 | C. jejuni NCTC 11168 with cat cassette in the kpsC gene; Cmr | This study |
| 81-176 | Clinical isolate | 32 |
| 81-176 motA::cat | C. jejuni 81-176 with cat cassette in the motA gene; Cmr | This study |
| 81-176 motB::cat | C. jejuni 81-176 with cat cassette in the motB gene; Cmr | This study |
| 81-176 ΔpflA::cat | C. jejuni 81-176 in which pflA gene is deleted and replaced by a cat cassette; Cmr | This study |
| 81-176 ΔflhA::cat | C. jejuni 81-176 in which flhA gene is deleted and replaced by a cat cassette; Cmr | This study |
| M1 | Environmental isolate | D. Newell |
| M1 motA::cat | C. jejuni M1 with cat cassette in the motA gene; Cmr | This study |
| M1 motB::cat | C. jejuni M1 with cat cassette in the motB gene; Cmr | This study |
| M1 ΔpflA::cat | C. jejuni M1 in which pflA gene is deleted and replaced by a cat cassette; Cmr | This study |
| M1 ΔflhA::cat | C. jejuni M1 in which flhA gene is deleted and replaced by a cat cassette; Cmr | This study |
| Bacteriophages | ||
| Φ1 | UK typing phage 1, NCTC 12673 | 17 |
| Φ2 | UK typing phage 2, NCTC 12674 | 17 |
| Φ3 | UK typing phage 3, NCTC 12682 | 17 |
| Φ4 | UK typing phage 4, NCTC 12676 | 17 |
| Φ5 | UK typing phage 5, NCTC 12678 | 17 |
| Φ6 | UK typing phage 6, NCTC 12680 | 17 |
| Φ7 | UK typing phage 7, NCTC 12671 | 17 |
| Φ8 | UK typing phage 8, NCTC 12681 | 17 |
| Φ9 | UK typing phage 9, NCTC 12669 | 17 |
| Φ10 | UK typing phage 10, NCTC 12683 | 17 |
| Φ11 | UK typing phage 11, NCTC 12679 | 17 |
| Φ12 | UK typing phage 12, NCTC 12677 | 17 |
| Φ13 | UK typing phage 13, NCTC 12672 | 17 |
| Φ14 | UK typing phage 14, NCTC 12675 | 17 |
| Φ15 | UK typing phage 15, NCTC 12684 | 17 |
| Φ16 | UK typing phage 16, NCTC 12670 | 17 |
| Plasmids | ||
| pRY111 | Source of cat cassette | 60 |
| pAJG180 | pUC19 carrying PCR fragment of region upstream of NCTC 11168 flhA; Apr | This study |
| pAJG181 | pAJG180 carrying cat from pRY111; Apr Cmr | This study |
| pAJG182 | flhA deletion construct; pAJG181 carrying NCTC 11168 cj0881c PCR fragment 3′ to cat; Apr Cmr | This study |
| pAJG316 | pUC19 carrying NCTC 11168 kpsC gene; Apr | This study |
| pAJG317 | kpsC deletion-insertion construct; pAJG316 with cat cassette inserted into kpsC; Apr Cmr | This study |
| pJP1 | pUC19 carrying PCR fragment of NCTC 11168 region upstream of pflA; Apr | This study |
| pJP2 | pJP1 carrying PCR fragment of NCTC 11168 region downstream of pflA; Apr | This study |
| pBEG000 | pUC19 carrying PCR fragment of NCTC 1168 motA gene; Apr | This study |
| pBEG001 | pflA deletion construct; pJP2 with cat cassette inserted into the BamHI site; Apr Cmr | This study |
| pBEG003 | motA insertion construct; pBEG000 with cat cassette inserted into the NcoI site of motA; Apr Cmr | This study |
| pBEG004 | pUC19 carrying PCR fragment of NCTC 11168 motB gene; Apr | This study |
| PBEG005 | motB insertion construct; pBEG004 with cat cassette inserted into the XbaI site of motB; Apr Cmr | This study |
| pUC19 | Cloning vector; Apr | 59 |
a
Cmr, chloramphenicol resistance; Apr, ampicillin resistance; Kmr, kanamycin resistance.
b
-, kindly provided by B. Wren, A. Karlyshev, and G. Thacker, London School of Hygiene and Tropical Medicine. CRU, Campylobacter Reference Unit (Health Protection Agency, Colindale, London, United Kingdom).