TABLE 3.

Effects of enzymes, pH, and temperature on the activity of bacteriocin T8

Treatment	Bacteriocin activitya
Enzymes (0.5 mg/ml)
Amylase	+
Pepsin, pronase, or proteinase K	−
Control (not treated with enzymes, 30°C)	+
pH
2	−
4, 6, 8, or 10	+
12	−
Control (pH not adjusted, 30°C)	+
Heat
10, 30, or 90 min at 30°C	+
10, 30, or 90 min at 60°C	+
10 or 30 min at 100°C	+
90 min at 100°C	−
Control (bacteriocin at 4°C)	+

^a+, activity; −, no activity.