FIG. 5.

Expression analysis of the genes bcaba1-4 in wild-type strain ATCC 58025 as well as ΔBcaba3, ΔBcaba1, ΔBcaba2, and ΔBcaba4 mutants. Total RNA was extracted from mycelium grown for 4 days in Sprecher medium. PCR fragments of the respective genes were used for probing. Loading of lanes with RNA was checked by probing with rRNA genes. Because of the strong signals in some of the mutant lanes, exposure time had to be short; therefore, the wild-type lane shows rather weak signals (compare to Fig. 2).