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TatC-dependent processing of SpTorALipA fusions. SDS-PAGE (11% gel), Western blotting, and immunostaining with a rabbit antiserum against LipA were performed with cell lysates of E. coli HB2151 and E. coli HB2151ΔtatC transformed with pCANTABSpTorA-AQAATD-LipA (lanes 1 and 2, respectively) and cell lysates of E. coli HB2151 and E. coli HB2151ΔtatC transformed with pCANTABSpTorALipA (lanes 3 and 4, respectively).
