Detection of phage-bound LipA. (A) LipA-g3p fusion proteins in 0.25-μg phage suspensions were visualized by SDS-PAGE, Western blotting, and immunostaining with rabbit antisera against LipA (αLipA) (left panel) or mouse monoclonal antibodies against g3p (αg3p) (right panel). Phages were isolated from the growth media of E. coli TG-1 cells transformed with pCANTABSpBlaLipA (SpBla), pCANTABSpG3pLipA (SpG3p), or pCANTABSpTorALipA (SpTorA). (B) Hydrolysis of p-nitrophenyl caprylate by phage-bound LipA. +, hydrolysis; −, no hydrolysis.