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. 2006 Jul;72(7):4589–4595. doi: 10.1128/AEM.02750-05

FIG. 4.

FIG. 4.

Detection of CesA in the periplasmic fraction, spheroplasts, and whole-cell extract (20 μg of protein per lane): SDS-PAGE (12.5% gel), Western blotting, and immunostaining with a rabbit antiserum against CesA of the periplasmic fractions (A), spheroplasts (C), and whole-cell extracts (E) of E. coli HB2151 and E. coli HB2151ΔtatC transformed with pCANTABSpBlaCesA (lanes 1 and 2), pCANTABSpG3pCesA (lanes 3 and 4), or pCANTABSpTorACesA (lanes 5 and 6). Lanes 1, 3, and 5 contained samples from E. coli HB2151, and lanes 2, 4, and 6 contained samples from E. coli HB2151ΔtatC. (B) Hydrolysis of racemic esters of 1,2-O-ispropylidene-sn-glycerol butyrate. (D) Hydrolysis of the methyl ester of (S)-naproxen. +, enzymatic activity; ++, higher enzymatic activity; −, no enzymatic activity.