FIG. 5.

Detection of phage-bound CesA. (A) CesA-g3p fusion proteins in 0.25-μg phage suspensions were visualized by SDS-PAGE, Western blotting, and immunostaining with rabbit antisera against CesA (αCesA) (left panel) or mouse monoclonal antibodies against g3p (αg3P) (right panel). Phages were isolated from the growth media of E. coli TG-1 cells transformed with pCANTABSpBlaCesA, pCANTABSpG3pCesA, or pCANTABSpTorACesA. (B) Hydrolysis of the methyl ester of (S)-naproxen by phage-bound CesA. +, enzymatic activity; −, no enzymatic activity.