FIG. 3.

Identification of a macromolecule, reacting with antibodies against OA. Extracts were prepared from tissue of S. domuncula and separated by 0.1% SDS-10% PAGE (PAGE). The crude extract was size separated and stained with Coomassie brilliant blue. In a parallel series of experiments, the proteins were blot transferred, and the Western blots (WB) were reacted with PcAb-OA (aOA; lane a) or PcAb-aGAL (aGal; lane b). In both blots a protein with a molecular weight of 35,000 was recognized. In addition to immune sera (i.s.), preimmune sera (pi.s.; lanes c and d) were also applied. Size markers are given.