FIG. 3.

Steady-state intracellular metabolite concentrations for P. chrysogenum before and after the switch to the gluconate-containing feed. Broth samples used for metabolite analysis were harvested after about 4 and 8 residence times. The standard deviations (indicated by error bars) were fixed at 10%. A metabolite whose concentrations were significantly different before and after addition of gluconate was identified by using a two-tailed equal variance t test and is indicated by an asterisk. A P value less than 0.01 was considered significant. Glyox, glyoxylate; PYR, pyruvate; FUM, fumarate; MAL, malate; a-KG, α-ketoglutarate; G1P, glucose-1-phosphate; G6P, glucose-6-phosphate; F6P, fructose-6-phosphate; M6P, mannose-6-phosphate; T6P, threhalose-6-phosphate; PEP, phosphoenolpyruvate; 2/3PG, combined pool of 2-phosphoglycerate and 3-phosphoglycerate; FBP, 1,6-fructose bisphosphate; 6PG, 6-phosphogluconate.