TABLE 2.

Primers and probes used for quantitative real-time PCR

Primer or probe	Sequence
Primers
gusA-1257F	5′-AGGTGCACGGGAATATTTCG-3′
gusA-1317R	5′-ACGCGTCGGGTCGAGTT-3′
nifA1-933F	5′-CGGCCGGTTCGAATTG-3′
nifA1-992R	5′-GAAATCTCGCCGATTTCATCA-3′
nifA2-122F	5′-TCCCTATTGTGCCGTTGCA-3′
nifA2-185R	5′-GCGGTGAGCGCTATCGA-3′
nifH-602F	5′-CCGCCAGACTCAATTCCAA-3′
nifH-662R	5′-GCGTGCTGGACGATGTT-3′
acdS-299F	5′-GCTGGGTTCCACATGAGGAT-3′
acdS-365R	5′-CCCAAGATGCGGCTCAAG-3′
TaqMan probes
gusA-1279TM	5′-CCACTGGCGGAAGCAACGCG-3′
nifA1-950TM	5′-CGAATGGCGGAACCCTGCTGC-3′
nifA2-143TM	5′-AGAGGGCGCCGGACATCTTTGAAAC-3′
nifH-625TM	5′-ATCCACTTCGTGCCGCGC-3′
acdS-323TM	5′-TCTACGACCGGGTCGGCAACATTC-3′