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Production of l-cysteine plus l-cystine by E. coli strain JM39ΔtnaA with pACYC-M256I carrying the altered cysE gene and plasmids carrying drug transporter genes after 48 h of cultivation. Detailed procedures for culture are described in the text. The concentration of l-cysteine plus l-cystine was determined by a microbioassay. Values indicate means and standard deviations of results from three independent experiments.
