TABLE 1.
PCR oligonucleotides designed and used for cloning the cry1Ia7 gene
| Primer | Sequence (5′-3′) | Positions | Sourcee |
|---|---|---|---|
| 1I(98)Fw | CACTAAAAAATGAAACAGATATAGA | 74-98a | Van Rie, pers. comm. |
| 1I(98)Rv | CCACATATTCATATACTGAGTGRTT | 1057-1080a | Van Rie, pers. comm. |
| 1Ia(10)Fw | TGTCTGAGTATGAAAATGTAGA | 134-155c | Van Rie, pers. comm. |
| 1Ia(11)Rv | GTTTTAATTGGATACATTTG | 841-860c | Van Rie, pers. comm. |
| 1Ib(8)Fw | TCTGAGCATGAGAGTATTGA | 136-155b | Van Rie, pers. comm. |
| 1Ib(9)Rv | GTGGTTTTAATAGGATATACAA | 842-862b | Van Rie, pers. comm. |
| 1I(A)Fw | GTATGAATAAAATTATATCTG | 300-320c | This study |
| 1I(B)Rv | GCAACAAATGTAAATTTGCAGC | 928-949c | This study |
| 1I(C)Fw | TTCTCTACCATAGAGTCTGC | 1318-1337c | This study |
| 1I(D)Rv | TTATAGTCTAAGTCCTCTCC | 2134-2153c | This study |
| 1I(E)Rv | CTACATGTTACGCTCAATATGGA | 2138-2160a | This study |
| 1I(F)Rv | GCAGACTCTATGGTAGAG | 1320-1337c | This study |
| 1I(G)Fw | GGTCTAAATAACTTGAGGGG | 1078-1097c | This study |
| 1I(H)Rv | GAAGAGAAGTTCCAAGCACC | 2221-2240c | This study |
| MUTFw | GGGCTAGCATGAAACTAAAGAATCCd | 1-17a | This study |
a
The positions given correspond to the cry1Ia7 gene.
b
The positions given correspond to the cry1Ib1 gene.
c
The positions given correspond to the cry1Ia1 gene.
d
The underlined nucleotides represent the NheI site.
e
Van Rie, pers. comm., personal communication with J. Van Rie (Bayer, Belgium).