Percent identity among mature peptides predicted from cDNA (A) and schematic alignment (B) of glyoxal oxidase (glx) and six new copper radical oxidases (cro1 through cro6). Sequences were aligned by ClustalW (38). Introns and exons are shown in white and black boxes, respectively. Residues involved in glyoxal oxidase catalysis are indicated, as are conserved positions within cro1 though cro6. Repeats of the highly conserved WSC domain, potentially involved in carbohydrate binding (IPR288; www.ebi.ac.uk/interpro/), are indicated by arrows underlying cro3, cro4, and cro5. The genes are arbitrarily aligned at the His471 position.