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Effect of lysosomal and proteasomal inhibitors on E208K Cx32 degradation. E208K Cx32-expressing cells were incubated for 6 h at 37°C in either the absence (A) or presence (B–I) of 20 μg/ml cycloheximide with the following additions: 100 μM ALLN (C), 20 μM Cbz-leu-leu-leucinal (D), 10 μM lactacystin (E), 20 μM ZL3VS (F), 100 μg/ml leupeptin (G), 200 μM chloroquine (H), or 100 μM ALLM (I).
